Abstract
In this study a mapping population (F8) of ca 200 progeny from a cross between the commercial rice varieties Apo and IR64 has been both genotyped and phenotyped. A genotyping-by-sequencing approach was first used to identify 2,681 polymorphic SNP markers which gave dense coverage of the genome with a good distribution across all 12 chromosomes. The coefficient of parentage was also low, at 0.13, confirming that the parents are genetically distant from each other. The progeny, together with both parents, were grown under irrigated and water restricted conditions in a randomised block design. All grain was harvested to determine variation in yield across the population. The grains were then polished following standard procedures prior to performing the phenotyping analyses. A Gas Chromatography—Mass Spectrometry approach was used to determine the volatile biochemical profiles of each line and after data curation and processing, discriminatory metabolites were putatively identified based on in-house and commercial spectral libraries. These data were used to predict the potential role of these metabolites in determining differences in aroma between genotypes. A number of QTLs for yield and for individual metabolites have been identified. Following these combined multi-disciplinary analyses, it proved possible to identify a number of lines which appeared to combine the favourable aroma attributes of IR64 with the favourable (higher) yield potential of Apo. As such, these lines are excellent candidates to assess further as potential genotypes to work up into a new variety of rice which has both good yield and good quality, thus meeting the needs of both farmer and consumer alike.
Highlights
Flavour and aroma are two of the most important factors influencing the quality of rice as perceived by the consumer
We have previously shown that the volatile metabolomes of Apo and IR64 grains contain many metabolites that associate with the sensory descriptors of each, in both positive and negative terms, and we have developed a panel of compounds that discriminate between the aromatic quality of IR64 and Apo (Calingacion et al, 2015)
IR64 and 213 recombinant-inbred lines (F5) derived from a cross between Apo and IR64 were planted at the Experimental Station of the International Rice Research Institute (IRRI), Philippines in the dry season of 2011
Summary
Flavour and aroma are two of the most important factors influencing the quality of rice as perceived by the consumer. The identification of compounds contributing to rice aroma, the factors affecting the aroma profile, as well as the genetic basis of its main components have been major research concerns. Such goals are limited to rice breeding programs and are of general relevance to research programs on many food crops where advanced instrumentation for the detection of volatile aromatic compounds is becoming more widely used (Hall et al, 2008)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
