Abstract
A fluorescent probe (probe 1) with selectivity and sensitivity for H2S and N2H4 has been developed. Probe 1 exhibits dual-excitation and dual-emission fluorescence response to H2S and fluorescence enhancement toward N2H4 with distinct naked-eye color changes. Moreover, probe 1 displayed low detection limits for H2S and N2H4 of 0.90 nM and 23.6 nM, respectively. The reaction mechanism of probe 1 toward H2S and N2H4 was established using 1H NMR and HR-MS spectroscopy. Additionally, probe 1 was used for monitoring and differentiating H2S and N2H4 in HeLa cells and displays good biocompatibility and low cytotoxicity.
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