Abstract
A method was described for permanent cell cultures of rat submandibular gland epithelial cells. Under sterile conditions, the capsule was removed and the gland minced through a large mesh sieve. The resultant clumps were allowed to settle and then were harvested and replated with 0.1 per cent Versene in a modified ring cylinder technique. This resulted in 3–4 cell epithelial colonies which were again harvested with 0.1 per cent Versene and replated as permanent cultures. Subculturing for the first five subcultures was performed using Versene without trypsin. The use of trypsin for initiating the cultures or the early subcultures resulted in fibroblast rather than epithelial cell survival.
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