Abstract
(1) In the histochemical method for adenosine triphosphatase, when magnesium and ATP are in equivalent concentrations, and sodium is present in the medium, staining occurs in the tissues. When sodium is omitted from this medium some structures—previously stained in the presence of sodium—fail to stain. (2) Ouabain inhibits staining when magnesium and ATP are equivalent and sodium is present in the medium. (3) The effects of calcium, potassium and sodium on staining with the proposed medium parallel the effects of these ions on activity of sodium-activated ATPase in vitro. (4) When magnesium ions and ATP are not equivalent in the substrate, sodium and ouabain fail to affect staining. (5) Certain tissue structures—such as the axonic nerve ending—which have been shown to contain sodium-activated ATPase in vitro, stain in the presence of sodium when magnesium and ATP are equivalent, but fail to stain when sodium is omitted from this medium.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
