Abstract
To try to contribute new information on the problem concerning red cell destruction in the spleen, a new method which makes it possible indirectly to quantitate H+-ion activity relating to packed erythrocytes, has been devised. The method is based on the chemical reaction: 5 I– + I O–3 + 6 H+ ⇄ 3 I2 + 3 H2O When blood is added to a solution of iodate and iodine in excess, the amount of iodine formed depends on the H+-ion quantity offered by the blood. The erythrocytes primarily act as H+ donors, secondarily as iodine acceptors. Acetone added primarily liberates iodine from the erythrocytes and subsequently acts as a solvent. The amount of dissolved iodine was determined by photometry. The method makes it possible to quantitate the H+-ion activity relating to packed erythrocytes in microvolumes of blood.
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