Abstract
The biogenesis of ribosomes and their coordination of protein translation consume an enormous amount of cellular energy. As such, it has been established that the inhibition of either process can extend eukaryotic lifespan. Here, we used next-generation sequencing to compare ribosome-associated RNAs from normal strains of Caenorhabditis elegans to those carrying the life-extending daf-2 mutation. We found a long noncoding RNA (lncRNA), transcribed telomeric sequence 1 (tts-1), on ribosomes of the daf-2 mutant. Depleting tts-1 in daf-2 mutants increases ribosome levels and significantly shortens their extended lifespan. We find tts-1 is also required for the longer lifespan of the mitochondrial clk-1 mutants but not the feeding-defective eat-2 mutants. In line with this, the clk-1 mutants express more tts-1 and fewer ribosomes than the eat-2 mutants. Our results suggest that the expression of tts-1 functions in different longevity pathways to reduce ribosome levels in a way that promotes life extension.
Highlights
The aging of eukaryotes depends on a number of genetic and environmental factors
The biogenesis of ribosomes and their coordination of protein translation consume an enormous amount of cellular energy
We found a long noncoding RNA, transcribed telomeric sequence 1, on ribosomes of the daf-2 mutant
Summary
Essers et al find a long noncoding RNA, transcribed telomeric sequence 1 (tts-1), on ribosomes of C. elegans carrying the life-extending daf-2 insulin receptor mutation as well as the clk-1 mitochondrial mutant. They demonstrate that this RNA is required for the life-extension phenotypes and that its depletion results in increasing ribosome levels. Highlights d The long noncoding RNA tts-1 is found on C. elegans daf-2 mutant ribosomes d Depletion of tts-1 restores ribosome levels in daf-2 mutants d The extended lifespans of daf-2 and clk-1 mutants are dependent on tts-1.
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