Abstract
A novel biosensor for immunoglobulin G (IgG) detection based on an extended-gate type organic field effect transistor (OFET) has been developed that possesses an anti-IgG antibody on its extended-gate electrode and can be operated below 3 V. The titration results from the target IgG in the presence of a bovine serum albumin interferent, clearly exhibiting a negative shift in the OFET transfer curve with increasing IgG concentration. This is presumed to be due an interaction between target IgG and the immobilized anti-IgG antibody on the extended-gate electrode. As a result, a linear range from 0 to 10 µg/mL was achieved with a relatively low detection limit of 0.62 µg/mL (=4 nM). We believe that these results open up opportunities for applying extended-gate-type OFETs to immunosensing.
Highlights
Recent efforts in biomedical research have been focused on preventative healthcare and rapid diagnoses of incipient diseases [1]
We designed an immunosensor using an extended-gate-type organic field effect transistor (OFET), based on research work that assumes that immunoglobulin G (IgG) detection is performed in water
For the determination of the limit of detection (LOD) and the limit of quantitation (LOQ) toward the target IgG, the intersection of the minimum signal (=Y) and the regression line obtained from the value of (VTH − VTH0)/VTH0 in the dynamic range of the titration curve allowed us to estimate the values of the LOD and the limit of quantification (LOQ)
Summary
Recent efforts in biomedical research have been focused on preventative healthcare and rapid diagnoses of incipient diseases [1]. Many types of immunoassays, such as radioimmunoassay (RIAs), enzyme-linked immunosorbent assays (ELISAs), fluorescent and chemiluminescent immunoassays, have been developed, because of their specificity and sensitivity [3]. These immunoassays are relatively complicated, due to the necessity of labels; for example, RIAs and ELISAs need radioisotopic and enzymatic labels, respectively. We succeeded in developing an extended-gate-type OFET, within which the extended gate surface is functionalized with streptavidin for the specific detection of biotinylated-immunoglobulin G (IgG) [8]. The operation mechanism of extended-gate-type FET sensors can be explained by an interfacial potential shift at the gate/solution interface. The success of this research work allows us to propose a new extended-gate-type OFET immunosensor for IgG detection. We report on the immunosensing of unlabeled IgG (=target IgG) in an aqueous solution using an extended-gate-type OFET
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
