Abstract
A rapid and feasible method of HPTLC is standardized for quantification of anethole in essential oil’s extract and from herbal formulations of fennel seed. The developed densitometric HPTLC method was performed to estimate the existence of anethole in the essential oil, extract and herbal formulations of fennel with the optimized concentration of hexane: Ethyl acetate (8:2%, v/v, mobile phase) on glass coated silica gel 60 F254 plates (20 × 10 cm) scanned with the absorbance of λ260 nm under densitometric condition. The Linearity of regressions revealed a satisfactory relationship between peak area and concentration of anethole in between the range of 100-600 ng/spot. This reliable method was validated as per the ICH guidelines to fulfill the necessary parameters such as accuracy and robustness. The amount of anethole in essential oil (0.098 ± 0.002%), extract (0.101 ± 0.004%) and three herbal formulations A (0.024 ± 0.004%), C (0.019 ± 0.002%) while anethole is not detected in B formulations from fennel seed was completely estimated by the developed method. The standardized methods and its validation gave new insights of HPTLC based detection and quantification of anethole in other aromatic plants as well as in other pharmacological formulations.
Highlights
Aromatic plants, usually used for flavoring, seasoning and imparting aroma in foods having important classes of valuable metabolites showed different biological activities, e.g. antioxidant and antimicrobial activity
A GC/MS study revealed that anethole is the most abundant compound present in fennel essential oil [6]
We have to standardize the HPTLC method for Foeniculum vulgare to isolate and quantify the anethole to check the quality of herbal products present in market
Summary
Usually used for flavoring, seasoning and imparting aroma in foods having important classes of valuable metabolites showed different biological activities, e.g. antioxidant and antimicrobial activity. In other studies of fennel, the seed has shown the anticancer activity and along with used in respiratory disorder reported that [4,5]. A GC/MS study revealed that anethole is the most abundant compound present in fennel essential oil [6]. Due to adulteration or their geographical origin the active metabolites are either metabolites are not detected or found in low amount of anethole which affect their quality Due to this reason, we have to standardize the HPTLC method for Foeniculum vulgare to isolate and quantify the anethole to check the quality of herbal products present in market
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