Abstract
Successful pathogenesis requires a number of coordinated processes whose genetic bases remain to be fully characterized. We utilized a high-throughput, liquid media-based assay to screen transposon disruptants of the phytopathogen Pseudomonas syringae pv. maculicola ES4326 to identify genes required for virulence on Arabidopsis. Many genes identified through this screen were involved in processes such as type III secretion, periplasmic glucan biosynthesis, flagellar motility, and amino acid biosynthesis. A small set of genes did not fall into any of these functional groups, and their disruption resulted in context-specific effects on in planta bacterial growth.
Highlights
Forward genetic screens have provided valuable insights into the various regulatory elements, chemicals, and other cellular components required for the virulence of bacterial phytopathogens [1,2,3,4,5]
Our work provided novel insights into the nutritional requirements of Pma ES4326 in Arabidopsis and revealed genes not previously known to be associated with P. syringae virulence
Half of these hits involved genes associated with the type III secretion system (TTSS), including structural components, enzymes, and upstream regulatory elements that control hrp/hrc gene transcription
Summary
Forward genetic screens have provided valuable insights into the various regulatory elements, chemicals, and other cellular components required for the virulence of bacterial phytopathogens [1,2,3,4,5]. Maculicola ES4326 (Pma ES4326), which is virulent on both Arabidopsis and radish. We subsequently used our high-throughput assay to screen this collection in order to identify genes required for bacterial virulence. This type of large-scale survey has not previously been performed with Pma ES4326. Our work provided novel insights into the nutritional requirements of Pma ES4326 in Arabidopsis and revealed genes not previously known to be associated with P. syringae virulence
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