Abstract
A major challenge in the field of biosensing is high-throughput screening (HTC) of small molecules in complex biological media such as human serum. Two aspects are required to achieve the goal: (1) General assays for target-independent detection of different molecules specifically and sensitively; (2) the operation is able to be standardized with automation possibility. This work integrates the distinguishing features of aptamer and fluorescent techniques. Innovatively, a very easy method is designed to combine a long range diffusion triggered excitation and a great steric hindrance effect, which makes it possible to become a general sensing method. As a proof-of-principle, adenosine in undiluted serum was detected. A nice detection limit and linear relationship were obtained.
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