Abstract

A GC-MS Based Metabolic Profiling of Probiotic Lactic Acid Bacteria Isolated from Traditional Food Products

Highlights

  • IntroductionLactobacillus and Pediococcus is a grampositive facultative anaerobic bacterium found widely in fermented food products and can be investigated for the metabolites present in them by Gas chromatography and mass spectrophotometry (GC-MS) based metabolic profiling

  • Lactobacillus and Pediococcus is a grampositive facultative anaerobic bacterium found widely in fermented food products and can be investigated for the metabolites present in them by Gas chromatography and mass spectrophotometry (GC-MS) based metabolic profiling.Metabolic fingerprinting is explained as the semi-quantitative investigation of extracellular and intracellular metabolites, respectively (VillasBoas et al, 2005)

  • A GC-MS based metabolic profiling was carried out to study metabolic differences of lactic acid bacteria isolated from different food sources

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Summary

Introduction

Lactobacillus and Pediococcus is a grampositive facultative anaerobic bacterium found widely in fermented food products and can be investigated for the metabolites present in them by GC-MS based metabolic profiling. Metabolic fingerprinting is explained as the semi-quantitative investigation of extracellular (exo-metabolome) and intracellular (endometabolome) metabolites, respectively (VillasBoas et al, 2005). These metabolic profiles GC-MS has consistently been the most favoured analytical technique for the analysis of metabolites present in distinct biological samples. For the study of metabolites in the microbial sample, derivatization based GC-MS technique can be applied to identify the intracellular as well as extracellular metabolites of Lactobacillus and Pediococcus species

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