Abstract

A free cell suspension of Coffea arabica in a defined medium has been obtained. Growth studies were undertaken following dry weight, pH and conductivity of the culture in defined and undefined medium. The cultures were grown in both light and dark. The fatty acids (neutral lipids) of the coffee cell suspensions were studied under the above conditions and compared to the fatty acids found in the leaves, stems and bean of the coffee plant via gas chromatography. The fatty acids of the plant cells were found to be similar to those found in the leaves and stems and could be differentiated from the bean by the presence of linolenic acid.

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