Abstract
A simple method for the detection of Pseudomonas aeruginosa in bottled water and aqueous pharmaceutical and cosmetic preparations is described. Subsequent to enrichment in Thom et al.'s (1971) nitrofurantoin broth at 42 °C, streaking positive cultures on to King et al.'s (1954) agar with nitrofurantoin added and incubating at 42 °C. Stabbing colonies into single, three 2 cm layer tubes (violet red bile dextrose agar/plain agar/SIM medium) and identifying strains by their non‐fermentative attack on glucose, motility, oxidase reaction and failure to produce H2S or indole.
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