Abstract

The objective of this study was to improve detection of the blood vessel network in placenta while simultaneously distinguishing maternal from fetal villi for image analysis. Therefore, we developed a technique for dual fluorescent labeling of endothelial cells and fetal trophoblast. Placentomes (d90 and d130 of pregnancy) were fixed (10% formalin or Carnoy's soln) and paraffin embedded. Following antigen retrieval (50mM glycine, 1mM EDTA, 0.05% Tween 20, pH 9.0; 120 C, 10 min), maternal and fetal blood vessels were identified by immunofluorescent labeling with an antibody (c/n:ab28364, ABCAM) against CD31 antigen, and CF 633 conjugated IgG (c/n:20122, Biotium). The trophoblast layer was stained with FITC labeled BS1 lectin (c/n:FL‐1101; Vector; 20 μg/ml). CD31 labeling resulted in bright fluorescent localization of all blood vessels. Positive lectin staining of fetal trophoblast allowed differentiation of fetal and maternal compartments. Thus, CD31 staining along with fluorescently labeled lectin combined with microscopy and imaging techniques provide an excellent tool to study angiogenesis and vascular architecture in sheep placenta, and other tissues. Grants: Hatch ND1748; USDA‐NIFA 2011–67016‐30174.

Full Text

Published Version

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.