Abstract
Orthoclone OKT3 is a monoclonal antibody used in the treatment of transplant rejection. The development of anti-OKT3 antibodies after therapy with this drug is a side effect which must be monitored. The current ELISA test to detect these antibodies is difficult to standardize between laboratories and does not lend itself to frequent monitoring of small numbers of samples. This prompted us to develop a new method employing flow cytometry to detect the development of anti-murine antibodies. Comparing samples tested by both techniques, we found the flow cytometric method to be more sensitive and as specific as the ELISA methodology. This technique is adaptable to numerous serologic assays and could greatly expand the use of flow cytometry in the clinical laboratory.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
