Abstract
A first integrated linkage map of Acca sellowiana (Berg.) Burret (2n=2x=22), commonly known as ‘pineapple guava’ was constructed using a 160 (TCO×BR) full-sib F1 progeny based on intersimple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. To develop an integrated map, we used a novel statistical approach that provided a simultaneous estimation of linkage and linkage phases based on maximum-likelihood analysis. The integrated map comprised 219 (27 ISSRs and 192 AFLPs) linked markers that were assigned to 10 major linkage groups (LG) representing a total map length of 2875cM and average distance between markers of 13.8cM. TCO (14 LG, 83 markers, 1264.6cM) and BR (18 LG, 94 markers, 1295.1cM) parent maps were also constructed through the same statistical approach but using only appropriate pseudo-testcross configuration markers. All the LG of the integrated map shared markers with either TCO or, BR or both parental maps. The integrated map provides a framework for further genetic and genomic analyses in A. sellowiana.
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