Abstract
espanolLa extraccion de ADN de alta calidad de hongos puede ser afectada debido a la presencia de una pared celular compleja, alto contenido de polisacaridos o metabolitos secundarios. En esta investigacion se adapto un simple metodo de extraccion y purificacion de ADN de hongos filamentosos aislados del suelo, para aplicaciones tipo Polymerase Chain Reaction (PCR). La metodologia descrita es rapida y de bajo costo. EnglishExtraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.
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