Abstract

A 29-year-old G1 woman presented at 280 weeks after falling onto her abdomen. A Kleihauer-Betke test result was positive with 0.9% to 1.4% fetal cells (Figure 1, arrows; original magnification ×50), corresponding to a 54-mL fetal-maternal hemorrhage (FMH). Fetal monitoring with non-stress tests and fetal anemia studies remained normal. Thus, flow cytometry was performed confirming the presence of maternal fetal hemoglobin (5.8% HbF), but no fetal erythrocytes (Figure 2A; 50 000 red blood cells [RBC] stained with anti-fetal hemoglobin with phycoerythrin [anti-HbF PE], enabling separation of HbF-containing maternal erythrocytes from fetal erythrocytes.

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