Abstract
Microarray is the most powerful technology in the field of molecular diagnostics and genomics. The manufacturing of microarray devices, based on traditional materials such as silicon and glass, is not easily adaptable to integration into microfluidics system. Recently, there was an increased interest in using polymers as substrates for microarray devices production, but fabrication of microarray on polymer surfaces requires complicated chemistry modification. In this paper we report a simple and effective chemical process to generate an epoxy–silane coating on cyclic olefin polymer surface for the grafting of single-stranded DNA (ssDNA) probes. We demonstrated the effectiveness of this process by direct hybridization of microarray probes with oligonucleotides perfect matches and PCR product. All hybridized probes showed high values for signal-to-noise ratio with a negligible noise level. In addition we propose the water contact angle as process control parameter to control the chemical process capability for industrialization purpose.
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