Abstract
Hypochlorous acid (HClO) and sulfur dioxide (SO2) play vital roles in various fields, including living organisms, water samples, food and so on. High doses of SO2 and HClO are seriously harmful to human and are associated with a variety of diseases. Therefore, monitoring the level of SO2 and HClO in various fields is significant for exploring their roles. In this work, a novel dual-response fluorescent probe RPT for detecting SO2 and HClO is successfully designed and synthesized, which is composed by pyrylium moiety and thiazine part. The fluorescent probe RPT can respectively monitor SO2 and HClO by two well-separated emission wavelength with an obvious difference of 85 nm. The fluorescent probe RPT also displays desired properties such as simple synthetic method, high sensitivity and selectivity. In addition, as a biocompatible molecule, RPT has been successfully used for monitoring SO2 and HClO in HeLa cells and RAW 264.7 cells in two different channels (red channel and green channel). More importantly, SO2 and HClO levels in various water and food samples have been detected with satisfactory results.
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