Abstract
Gastric cancer (GC) is the TOP3 leading cause of human mortality in malignant tumors. Notwithstanding, the association between GC and circRNAs is not clear. The purpose of this research was to determine the association between GC progression and circRNAs. The data of circRNAs was obtained from the Gene Expression Omnibus (GEO) database to identify gene, which differentially expressed circRNAs in GC tissues and paired normal tissues. The expression of circRNAs in cancer tissues and normal tissues were tested, and the target circRNA was verified before and after surgery in the plasma. A circRNA-micro(mi)RNA-mRNA competing endogenous RNAs (ceRNAs) network was established, and GO and KEGG analysis are performed. Five candidate circRNAs were identified through bioinformatics analysis. Hsa_circ_0021087 and hsa_circ_0005051 were both downregulated in GC tissues, cells and plasma by RTq-PCR. Additionally, there was a significant difference in the expression of plasma hsa_circ_0021087 in patients with GC at the preoperative and postoperative stages (P < 0.001). Hsa_circ_0021087 also promoted the proliferation of GC cells in vitro. Next, the circRNA-miRNA-mRNA network of hsa_circ_0021087 was predicted, which may be associated with the development of GC by bioinformatics analysis. In summary, the aforementioned dual-circular RNAs may have important implications on the potential, novel and non-invasive diagnostic method for patients with GC.
Highlights
Gastric cancer (GC) has become the third leading cause of cancer-associated death and is the 6th common cancer in the world [1]
The expression levels of five candidate circRNAs were verified by RTq-PCR in 70 GC samples and 19 normal blood samples
The results indicated that both hsa_circ_0021087 and hsa_circ_0005051 were downregulated in GC (P < 0.01; Figures 2A,B), which was consistent with the results of the microarray analysis
Summary
Gastric cancer (GC) has become the third leading cause of cancer-associated death and is the 6th common cancer in the world [1]. In order to improve the early diagnosis, treatment and prevention of GC, the discovery of new molecular biomarkers and therapeutic targets is significant. With the discovery of more and more circRNAs, the function of circRNAs has been gradually reported, especially to promote the physiological and pathological progression of disease, such as cell proliferation, apoptosis, CircRNA as a Diagnostic Biomarker for Gastric Cancer migration and carcinogenesis [5]. In the past few years, studies have demonstrated that circRNAs are involved in many malignancies, such as gastric cancer [6], hepatocellular carcinoma [7], colon cancer [8], and pancreatic carcinoma [9], by acting as miRNA sponges and inhibiting target gene expression
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