A Disposable Membrane Strip Immunosensor in Fresh Produce Application

As the safety in the food supply becomes critical, the demand for rapid, low volume, sensitive, and economic biosensing devices has dramatically increased. An immunosensor based on electrochemical sandwich immunoassay using polyaniline has been developed for detecting foodborne pathogens, such as Escherichia coli 0157:H7. The immunosensor design is based primarily upon the specific nature of labeled antibody-antigen binding. The architecture of the biosensor demonstrates the advantages of using lateral flow formal strip attached to a portable circuitry for signal measurement. The ability to change the specificity of the antibodies enables the biosensor to be used as a semi-quantitative detection device for other types of foodborne pathogens. Results show that the biosensor could detect as low as 10/sup 1/ bacterial cells per milliliter in less than 10 minutes. Additionally, the biosensor yields faster results, and is cheaper than conventional approaches. Details on the device fabrication and performance of the biosensor in detecting E. coli 0157:H7 are presented.

A conductometric biosensor for biosecurity

A Disposable Biosensor for Pathogen Detection in Fresh Produce Samples

Purpose of Review This editorial review aims to provide readers with an introduction to the Current Clinical Microbiology Report Special Issue “Foodborne Pathogens” and to point out overarching novel trends and developments in this field. Recent Findings The first global burden of foodborne disease study by the WHO estimated that foodborne diseases annually cause illness in 1 in 10 people, resulting in 420,000 deaths per year. In recent years, the threat of foodborne antimicrobial-resistant pathogens has intensified, facing researchers and authorities with major challenges. Whole genome sequencing, MALDI-TOF mass spectrometry, and Fourier transform infrared spectroscopy enable improved detection and typing of foodborne pathogens. Summary Foodborne diseases remain a major risk to global health. At the same time, crucial advances in the development of technological platforms have led to unprecedented opportunities in detection, typing, and source attribution of foodborne pathogens.

Efficacy of electrolyzed oxidizing (EO) and chemically modified water on different types of foodborne pathogens

The paper select instant frozen dumplings to study the quality and safety of the quick-frozen food supply chain. And analysis each quality and safety risk factors on the supply chain of instant frozen dumpling, determine the weight of each factor by using AHP. Empirical research on quality and safety of supply chain was carried out in a Chinese frozen dumpling enterprise. On this basis, some recommendations were put forward to enhance the quality and safety of supply chain in instant frozen dumpling enterprise.

This paper aimed to revisit the literature on food safety in global supply chains and analyze the evolution and contemporary challenges in this research field. It conducted a systematic literature review in two steps based on a selection of 178 articles. It analyzed safety in food supply chains using the Six T's Framework to evaluate food safety management. In the supply chain literature, traceability was the most studied element of the original framework. The research identified studies that have incorporated the topics of "tactics" and "targets" in their analysis of safety in supply chains. This article presented an expanded framework, a tool for categorizing research, and areas for advancement in the identification of quality indicators in global food chains for the current research agenda. The paper contributed theoretically to the discussion of safety elements in food supply chains and incorporated new elements into the originally conceived framework. Thus, it helped to delineate the research field and opened up an agenda for future research.

As the safety in the food supply becomes critical, the demand for a rapid, low-volume, and sensitive microbial detection device has dramatically increased. A biosensor based on an electrochemical sandwich immunoassay using polyaniline has been developed for detecting foodborne pathogens, such as Escherichia coli (E. coli) O157:H7. The biosensor is comprised of two types of proteins: capture protein and reporter protein. The capture protein is immobilized on a pad between two electrodes, while the reporter protein is attached to conductive polymers. After adding the sample, the target protein binds to the reporter protein and forms a sandwich complex with the capture protein. The conductive polymer that is attached to the reporter protein serves as a messenger, reporting the amount of target protein captured in the form of an electrical signal. The architecture of the biosensor utilizes a lateral flow format, which allows the liquid sample to move from one pad to another by capillary action. Experiments to evaluate the best construction materials, the optimal polyaniline and antibody concentrations, and the distance between electrodes are highlighted in this paper. Results show that the biosensor could detect approximately 7.8/spl times/10/sup 1/ colony forming unit per milliliter of E. coli O157:H7 in 10 min.

A paramount and alluring sphere of research, now-a-days, is food analysis, because of the breakneck augmentation of food enterprise and highly hightened maneuverability of today's populations. The management of food quality is very indispensable both for consumer safeguard as well as the food corporations. The biosensors' application in the field of food analysis is quite propitious for the revealing of food borne pathogens. Biosensor, an analytical device, transforms a biological response into an electrical signal. Bioreceptors and transducers are the two main components of a biosensor. Bioreceptor or biorecognition element is the one which leads to the recognition of target analyte and a transducer, for the conversion of recognized event into a measurable electrical signal. The development of biosensors improved the sensitivity and selectivity of detection techniques for food borne pathogens and is rapid, reliable, effective and highly suitable when used in in situ analysis. Since the security in the food supply becomes crucial because of increased perception among consumers and vying nature of food industries, the necessity for expeditious, low volume and sensitive biosensor devices has productively increased. TM Nevertheless , till date, a very few biosensor systems are available commercially such as Biacore, Spreeta , Reichert SR 7000, Analyte 2000, RAPTOR etc. Since, there is ever growing concern regarding safe food and water supply, it is very obvious that the demand for rapid detecting biosensors will also be increasing at par.

Herbal and plant extracts are being applied to a wide range of foods against different types of foodborne pathogens. In the present study, ethanolic and aqueous extracts of different concentrations (5% v/v, 10% v/v, and 20% v/v) from cranberry (Vaccinium macrocarpon), black chokeberry (Aronia melanocarpa), and pomegranate (Punica granatum L.) plants were applied in five concentrations (62.5 to 1000 mg/mL) against foodborne strains of Staphylococcus aureus, Escherichia coli, and Streptococcus pyogenes. The results revealed that a low concentration of solvents (5% v/v) did not exhibit decreased antimicrobial activity in comparison with higher solvent concentrations (10% and 20% v/v). Additionally, both aqueous and ethanolic extracts were highly effective against pathogens even in their low concentrations (62.5 mg/mL and 125 mg/mL). Likewise, the extracts exhibited promising results (aqueous extracts of pomegranate, cranberry, and black chokeberry in a food-compatible concentration of 2% w/v) were applied to raw pork meatball production, and their antimicrobial activity was recorded versus Enterobacteriaceae, total mesophilic bacteria (TMB), lactic acid bacteria (LAB), Staphylococcus spp., Pseudomonas spp., and yeasts/molds. The outcome demonstrated that meatballs that contained aqueous extracts of pomegranate were more resistant to spoilage compared to all of the other samples, as it was preserved for more days. Likewise, these extracts of a plant origin could be used as natural preservatives in meat products, even in their low concentrations.

Multiplex bacteria detection using one-pot CRISPR/Cas13a-based droplet microfluidics

Development of a random genomic DNA microarray for the detection and identification of Listeria monocytogenes in milk

The market size of the home meal replacement (HMR) is continuously increasing. However, there are high concerns about safety such as poor hygiene of companies and detection of foodborne pathogens in HMR. This study analyzed the contamination level of total aerobic bacteria and 5 types of foodborne pathogens in 30 Ready-to-Eat foods (20 Gimbap, 5 sandwiches and 5 hamburgers). As a result, it was confirmed that total aerobic bacteria were contaminated with at least 67 CFU/g and up to 412 CFU/g in 12 samples. Staphylococcus aureus (11-132 CFU/g) and Bacillus cereus (41-233 CFU/g) were contaminated in 9 samples. The microbial contamination level was the highest in Gimbap. Small amounts of foodborne pathogens can multiply during inappropriate distribution and storage, causing foodborne illness. Therefore, to expand and growth of HMR industry further, it is considered necessary to secure microbiological safety through continuous monitoring of HMR and hygiene management of companies.

Bioactive compounds and organic acids are applied to a wide range of foods against different types of foodborne pathogens. In the present study, carvacrol and thymol (1000 mg/L) were applied in wine-based marinades, alone or in combination with them and in combination with tartaric acid, malic acid, ascorbic acid, citric acid, and acetic acid (in concentration 0.1% w/v), in chicken and beef fillets and their antimicrobial activity, antioxidant capacity, and pH were estimated during refrigerated storage. Likewise, their antimicrobial activity was recorded against Enterobacteriaceae, total mesophilic bacteria, yeasts/molds, and lactic acid bacteria. The outcome demonstrated that both meats kept under similar storage conditions (4 °C/9 days) exhibited lower microbial growth, particularly with Enterobacteriaceae, when treated with wine-based carvacrol-thymol marinades and may extend their shelf-life. This antimicrobial action was more pronounced in the beef samples. The total phenolic content (TPC) and the antioxidant activity of the applied marinades were determined using the Folin-Ciocalteau method and ABTS and DPPH radical scavenging activity methods, respectively. The results revealed that marinades with thymol and/or carvacrol in combination with acetic or ascorbic acid had greater TPC and antioxidant activity. The pH values of the respective marinades applied to both chicken and beef fillets exhibited an upturn during storage. Consequently, these marinades, even at low concentrations, could be used as natural preservatives in meat products.

Nucleic acid testing is increasingly being utilized in the detection of foodborne pathogens. The implementation of fully automated and enclosed nucleic acid testing systems has the potential to significantly reduce manual labor and testing time for operators. Therefore, the development of such systems has become a focal point in current research. In this study, the focus is on addressing how to achieve fully automated detection at low cost. The research is primarily centered on the design of key functional modules required for the detection process including nucleic acid extraction, heating, and optical detection. The detection process does not require manual supervision or participation and does not require professional technical personnel, thereby reducing the usage cost. By simply adding a sample, it can simultaneously screen and analyze different types of foodborne pathogens, making it advantageous for the analysis and detection of complex samples. The design and manufacturing of the device are cost-effective and highly applicable. By adjusting the reaction program, the fully automated nucleic acid detection device can achieve compatibility with various amplification reactions, demonstrating its broad application prospects in the field of Cronobacter sakazakii analysis.