A direct method for measurement of gross surface area of mammalian gastro-intestinal tracts

Abstract

In early literature, gastro-intestinal tracts (GIT) are described with subjective terms such as ‘large’, ‘small’, ‘long’, ‘short’, or ‘capacious’ (e.g. Boker, 1932; Hill, 1958). Comparison of the size of the GIT of a particular species requires a reliable quantitative method. Length of the small intestine, caecum and colon has been used by ornithologists interested in GIT morphology (e.g. Davis, 1961; Levin, 1963; Moss, 1972, 1974; Ankey, 1977; Pulliainen, 1981; Pulliainen et al , 1981; Pulliainen and Tunkkari, 1983) and by a few researchers in mammalogy (Barry, 1977; Schieck and Millar, 1985; Woodall, 1987), but length does not reliably indicate volume and, therefore, the capacity of a GIT. Measurements of volume have been difficult to obtain. Chivers and Hladik (1980) attempted to measure volume of GIT regions using three different techniques. Filling a gut region with water was deemed inaccurate because of the tendency for GIT tissue to expand and, thus, give an inaccurate measurement. A second method, used for the stomach only, relied on a mathematical estimation based upon the length of the greater curvature in which this length was assumed to be equal to the circumference of a sphere. The third method relied on measurements of surface area for the stomach, which was assumed to be a sphere, and measurements of lengths and widths for the small intestine and colon, which were assumed to be cylinders. In their paper, the volume data were reported by use of the latter method. Chivers and Hladik (1980) recognized the potential problems concerned with the inaccuracy of these measurements.