Abstract
BackgroundThe cabbage beetle Colaphellus bowringi Baly is a serious insect pest of crucifers and undergoes reproductive diapause in soil. An understanding of the molecular mechanisms of diapause regulation, insecticide resistance, and other physiological processes is helpful for developing new management strategies for this beetle. However, the lack of genomic information and valid reference genes limits knowledge on the molecular bases of these physiological processes in this species.ResultsUsing Illumina sequencing, we obtained more than 57 million sequence reads derived from C. bowringi, which were assembled into 39,390 unique sequences. A Clusters of Orthologous Groups classification was obtained for 9,048 of these sequences, covering 25 categories, and 16,951 were assigned to 255 Kyoto Encyclopedia of Genes and Genomes pathways. Eleven candidate reference gene sequences from the transcriptome were then identified through reverse transcriptase polymerase chain reaction. Among these candidate genes, EF1α, ACT1, and RPL19 proved to be the most stable reference genes for different reverse transcriptase quantitative polymerase chain reaction experiments in C. bowringi. Conversely, aTUB and GAPDH were the least stable reference genes.ConclusionThe abundant putative C. bowringi transcript sequences reported enrich the genomic resources of this beetle. Importantly, the larger number of gene sequences and valid reference genes provide a valuable platform for future gene expression studies, especially with regard to exploring the molecular mechanisms of different physiological processes in this species.
Highlights
Insects in a diapause status may have a stronger resistance to environmental stresses, e.g., seasonal adverse conditions and pesticides [1,2]
Using Illumina sequencing, we obtained more than 57 million sequence reads derived from C. bowringi, which were assembled into 39,390 unique sequences
Eleven candidate reference gene sequences from the transcriptome were identified through reverse transcriptase polymerase chain reaction
Summary
Insects in a diapause status may have a stronger resistance to environmental stresses, e.g., seasonal adverse conditions and pesticides [1,2]. The cabbage beetle Colaphellus bowringi Baly is a serious insect pest of crucifers in mountain areas in China [3]. This beetle can survive during seasonal adverse conditions under a reproductive diapause status in soil [4,5]. A lack of genomic information and valid reference genes limits our understanding of the molecular bases of these physiological processes in efforts to develop new management strategies for this species. The cabbage beetle Colaphellus bowringi Baly is a serious insect pest of crucifers and undergoes reproductive diapause in soil. An understanding of the molecular mechanisms of diapause regulation, insecticide resistance, and other physiological processes is helpful for developing new management strategies for this beetle. The lack of genomic information and valid reference genes limits knowledge on the molecular bases of these physiological processes in this species
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