Abstract
Polyphenol-rich foods are part of many nutritional interventions aimed at improving health and preventing cardiometabolic diseases (CMDs). Polyphenols have oxidative, inflammatory, and/or metabolic effects. Research into the chemistry and biology of polyphenol bioactives is prolific but knowledge of their molecular interactions with proteins is limited. We mined public data to (i) identify proteins that interact with or metabolize polyphenols, (ii) mapped these proteins to pathways and networks, and (iii) annotated functions enriched within the resulting polyphenol-protein interactome. A total of 1,395 polyphenols and their metabolites were retrieved (using Phenol-Explorer and Dictionary of Natural Products) of which 369 polyphenols interacted with 5,699 unique proteins in 11,987 interactions as annotated in STITCH, Pathway Commons, and BindingDB. Pathway enrichment analysis using the KEGG repository identified a broad coverage of significant pathways of low specificity to particular polyphenol (sub)classes. When compared to drugs or micronutrients, polyphenols have pleiotropic effects across many biological processes related to metabolism and CMDs. These systems-wide effects were also found in the protein interactome of the polyphenol-rich citrus fruits, used as a case study. In sum, these findings provide a knowledgebase for identifying polyphenol classes (and polyphenol-rich foods) that individually or in combination influence metabolism.
Highlights
The process was designed as a KNIME workflow53
Chemical Entities of Biological Interest (CHEBI) structural entries [90,129] were identified in the DNP but 6,827 did not have chemical structures and were removed leaving 83,302 compounds. Chemical structures from both CHEBI and DNP polyphenol lists were standardized based on the following scheme: (i) remove fragments, (ii) neutralize, (iii) remove explicit hydrogens, (iv) tautomerize, and (v) aromatize
The list comparison was done by matching SMILES (Simplified molecular-input line-entry system) strings using strict stereochemistry criteria
Summary
Over 250,000 entries with fully defined chemical structures from DNP2 25.1 (CRC) were imported into a local JChem (ChemAxon) chemical structure database. Chemical Entities of Biological Interest (CHEBI) structural entries [90,129] were identified in the DNP but 6,827 did not have chemical structures and were removed leaving 83,302 compounds. Chemical structures from both CHEBI and DNP polyphenol lists were standardized based on the following scheme: (i) remove fragments, (ii) neutralize, (iii) remove explicit hydrogens, (iv) tautomerize, and (v) aromatize. The list comparison was done by matching SMILES (Simplified molecular-input line-entry system) strings using strict stereochemistry criteria. CHEBI identifiers were assigned to 1179 DNP polyphenols, which was 3.2% of the total list. All chemical structure manipulations and data analysis were performed with JChem nodes in KNIME
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