Abstract
The activity of purified ovine, bovine, porcine, rabbit, rat, and equine pituitary FSH and PMSG were compared with that of human FSH in the hCG-augmented ovarian growth assay of Steelman and Pohley (S-P) and in a rat testes tubule tissue receptor assay (TRA). Each of the pituitary FSH fractions studied showed greater activity in the TRA than in the S-P assay. In the TRA, all response curves were parallel with the exception of that for equine pituitary FSH. Highly purified LH preparations of the same species as the FSH's tested, were also examined in the TRA. Ovine and bovine LH showed no detectable FSH activity, while LH from the other species examined showed low activity, which presumably was due to residual FSH contamination. PMSG was an exception in that it showed greater activity in the in vivo S-P assay than in the in vitro TRA. This unique relationship is attributed to the very long plasma half-life of PMSG compared to that of the other FSH fractions tested. The more general phenomenon of greater in vitro vs in vivo activity of the FSHs can be explained by the insensitivity of the TRA to factors of metabolism that influence complete expression of in vivo activity.
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