Abstract
An embryo cryopreservation technique was described using a single-step addition of 1,2 propanediol in freezing straws preloaded with sucrose then placed in an alcohol bath followed by a control rate freezer (Biocool) and then finally plunged into liquid nitrogen. The embryos were subsequently thawed by a one-step dilution of the cryoprotectant. This technique was found to be very effective for freezing at the 2PN stage. The aim of this study was to retrospectively determine how effective this technique is for freezing day 3 multi-cell embryos.
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