Abstract
Samples of whole blood or plasma from four ambisexual and two gonochoristic teleost species were investigated for the occurrence of 11β-hydroxytestosterone and 11-ketotestosterone. The steroids were identified and quantified by double-isotope derivative assays (DIDA) after initial separation and purification by thin-layer and paper chromatography. In all samples from the ambisexual fish 11β-OH-testosterone was found in respectable quantities, whereas 11-ketotestosterone could not be detected in these animals. In the gonochoristic winter flounder and Atlantic salmon, 11-ketotestosterone was the predominant androgen, and compared to the ambisexual fish the concentration of 11β-hydroxytestosterone was extremely low.
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