A comparative study of the cell cycles of nullipotent and multipotent embryonal carcinoma cell lines during exponential growth

Abstract

Three embryonal carcinoma (EC) cell lines F9, PCC4 Azal, and PCC3 N/1 with a common origin from the transplantable ascites teratoma OTT6050 were analyzed with regard to cell cycle characteristics during exponential growth in the undifferentiated state. The three lines, in the sequence mentioned, have been shown to represent successive stages in early normal mouse embryogenesis from morula to blastocyst, as reflected in their cell surface antigens. In the multipotent lines an increased intraclonal variability in intermitotic times was found when analyzing family trees. In the PCC3 N/1 line the distribution was almost bimodal. The increased intermitotic time variability was found to be represented in the G 1 period that varied from less than 1 hr up to about 6 hr in the PCC3 N/1 line, with a mean value of 3.4 hr. The G 1 period of the F9 line had a range of only 3.5 hr, with a mean value of 1.5 hr. The S-G 2 period was found to be very similar in length in all three lines.