Abstract
Assessment of the compatibility between the probiotic and prebiotic is identified as a critical step while developing synbiotic products. So a study was conducted to compare the inulin utilization potential of two Bifidobacterium animalis subsp. lactis strains for selecting a strain for the development of a lyophilized synbiotic product. The growth stimulatory effect of inulin was found to be more prominent on Bifidobacterium animalis subsp. lactis B420 than on Bifidobacterium animalis subsp. lactis Bb-12. So a synbiotic pair of Bifidobacterium animalis subsp. lactis B420 and inulin was selected for the development of the synbiotic product. The developed product had a high probiotic count of 6.5x108 cfu/g, showing a survival rate of 65%. On assessing the probiotic count of the developed product during storage, a marked reduction was observed on room temperature storage with the counts reducing to the level of less than five log cfu/g after 60 days. In contrast the count remained in the range of eight to nine log cfu/g even after 60 days of refrigerated storage. So it could be a suggested that considering the probiotic stability and viability problems pertaining to fermented dairy products, lyophilized products could be potential alternatives as matrices for the delivery of probiotics to humans. Keywords: Bifidobacterium animalis subsp. lactis Bb-12, Bifidobacterium animalis subsp. lactis B420, Inulin, Lyophilized powder, Synbiotics.
Highlights
Synbiotics, which exploit the synergistic relationship between its two constituents, the probiotics and prebiotics, are gaining wide popularity as a means of altering the gut microbiota towards a beneficial one
The ability of Bifidobacterium animalis subsp. lactis B420 and Bifidobacterium animalis subsp. lactis Bb-12 to utilize, inulin was determined by monitoring their growth in a semi-liquid minimal media (Table 1)
The growth stimulatory effect of inulin was more prominent in the case of Bifidobacterium animalis subsp. lactis B420, as a marked reduction in its mean generation time was observed compared to that of Bifidobacterium animalis subsp. lactis Bb-12
Summary
Synbiotics, which exploit the synergistic relationship between its two constituents, the probiotics and prebiotics, are gaining wide popularity as a means of altering the gut microbiota towards a beneficial one. Maintenance of viability of probiotics and selection of a synergistic prebiotic are considered as the major challenges for developing successful synbiotic products. The most widely documented probiotic Bifidobacterium strain, Bb-12 belongs to this species. Wide variations are observed among the members of the genus Bifidobacterium in their ability to utilize inulin. Development of freeze dried synbiotic products could be a better option in this direction. Considering these aspects two commercially available probiotic Bifidobacterium animalis subsp. Lactis Bb-12 were assessed for their ability to utilize the prebiotic inulin to develop a lyophilized symbiotic preparation using the selected synbiotic pair Considering these aspects two commercially available probiotic Bifidobacterium animalis subsp. lactis cultures namely Bifidobacterium animalis subsp. lactis B420 and Bifidobacterium animalis subsp. lactis Bb-12 were assessed for their ability to utilize the prebiotic inulin to develop a lyophilized symbiotic preparation using the selected synbiotic pair
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