Abstract
A compact and highly sensitive light-emitting diode-induced fluorescence detector (LED-FD) was constructed and evaluated. The LED excitation beam was restricted by a 200μm diameter pinhole on a 55μm thick lighttight film. The LED, excitation filter, pinhole, and capillary flow cell were placed tightly against each other without either optical lens or excitation fiber. The performance of the LED-FD was evaluated by flow injection analysis (FIA) of sodium fluorescein solution. The limit of detection (LOD) was determined as 0.75nM (SNR=3). Compared with our previous work, a 3.5-fold enhancement on the signal-to-noise ratio (SNR) was obtained. The system exhibited linear responses in the concentration range of 2–200nM (R=0.9993). The error of repeatability was within 2.0% R.S.D. on peak height in a ten-repeated injection of 5nM solution. The reproducibility between devices was within 2.2% R.S.D. on peak height of 10nM fluorescein solution. Finally, the LED-FD was compared with commercial fluorescence detector, and similar fluorescence signal intensity was obtained.
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