A column screening study: Validation of a RP-LC method for determination of teriflunomide with pentafluorophenyl stationary phase

Abstract

The research focuses on column screening in order to develop a novel validation method to separate Teriflunomide significantly using comparative and equivalent column selection studies. This gives stationary phase efficiency relative to height equivalent to a theoretical plate (HETP) (>16000) and symmetry factor (<2.0%). After extensive column chemistry and technique development, the advanced Pentafluorophenyl (PFP) column separates halogenated drugs better. Teriflunomide is fluorinated drug, hence PFP is optimal for analysis. For statistical data comparison, the same chromatographic method was developed and transferred to another YMC Pack Pro C18 column that gives almost the same results as PFP column in terms of carbon load, pore size, and surface area. The refined and validated method yielded equivalent results with two stationary phases using a single analysis procedure. Column screening, technique creation, transfer, and validation with comparison studies with correlation coefficients over 0.999 are unique. The statistical data from both columns meets ICH requirements. The sensitive, reliable, robust, and reproducible approach is easy to apply in regular laboratory analysis. Data analysis showed that the validation study with PFP is better (LoD - 0.0015 ppm) for column ageing due to smaller injection volume and amount loaded in the column, shorter run time, and cheaper analysis cost. This research helps with column selection and method transfer with diverse column specifications. With less than 2.0% of RSD, the column screening analytical method validation is linear, precise, accurate, rugged, and efficient.