A clinically relevant model of stroke using aged rats

Aurel Popa‐Wagner ,Flavia Semida Ghinea,Monica Grigore,Bogdan Capitanescu ,Leonard Radu Pinosanu ,Mircea Popescu-Driga ,Dirk M Hermann

doi:10.24238/13221-12-1-198

Aurel Popa‐Wagner , Flavia Semida Ghinea + Show 5 more

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https://doi.org/10.24238/13221-12-1-198

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Comorbidities like diabetes, arterial hypertension, or comorbidity factors such as hypercholesterolemia is common in elderly persons and are associated with higher risk of stroke. Since stroke afflicts mostly the elderly comorbid patients, it is highly desirable to test the efficacy of cell therapies in an appropriate animal stroke model. Animal models of stroke often ignore age and comorbidities frequently associated with aging, and this could be one of the explanations for unsuccessful bench-to-bedside translation of neuroprotective strategies. In order to mimic more closely the clinical condition, we have established a model of ischemic stroke in aged rats. Using this model, we showed that post-stroke angiogenesis is not impaired in the lesioned, aged brain and conclude that stroke in aged rodent models in reliable and more closely related to the human condition.

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Worldwide the incidence of cerebral ischemia is increasing due to unhealthy lifestyle, comorbidities and an increase in the number in the elderly population
Stress and sedentary lifestyle associated with hypercholesterolemia, arterial hypertension, diabetes, obesity, are common in elderly persons and are associated with higher risk of stroke [2]
Advanced age is a risk factor for ischemic stroke, most of the experimental studies investigating this pathological condition have been conducted n young animals who unlike human patients recover rapidly. This would suggest that it is better to use aged rodents to test the efficacy of treatments aimed at improving behavioral recuperation after cerebral ischemia

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Introduction

Worldwide the incidence of cerebral ischemia is increasing due to unhealthy lifestyle, comorbidities and an increase in the number in the elderly population. Free floating sections were incubated first with a mouse anti-rat endothelial cell antigen (RECA) (1:200, abcam, UK) followed by a donkey-anti-rabbit-FITC 1:3000, (Dianova, Hamburg, Germany). At 7 days post-stroke the blood vessel became tortuous and leaky allowing BrdUlabelled cells, presumably of hematogenous origin, to enter the infarcted area in great numbers [2B, arrowheads].

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