Abstract
Capillary electrophoresis amperometric detection (CE–AD) microchip was fabricated for separation and detection of DNA fragments. The chip was fabricated on glass substrate and microchannels were laid in PDMS mold. The capillary was filled with 5% polyacrylamide gel and separation of DNA fragments of different molecular weight was achieved by application of 100 V DC potential across sample and waste reservoirs made at the capillary ends. The amperometric detection (AD) system involved in-channel gold microelectrodes to analyze oxidation peak for adenine residues in DNA chain. This technique was used in resolving single as well as double stranded DNA fragments.
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