Abstract

Abstract An automated, flow-through, multi-aquarium system with precise temperature control and dosed inputs of water soluble toxicants has been built from biologically inert materials for long term effect-studies on aquatic organisms. Although especially constructed for effect-studies of oil (water soluble fraction) on eggs and larvae of marine fishes, the biotest system holds the potential of testing other water soluble toxicants on aquatic animals within a size range from 1 mg to 1 kg. In its current form, the biotest system has a capacity of about 120.000 eggs or 60.000 yolk-sac larvae of cod (Gadus morhua L.), divided between two test groups and one control group, each of four parallel aquaria. Special consideration has been given to essential steps and components in order to maximize continuity of operation, safety, and ease of handling of the system. The long term temperature variation of the system is within 0.05° C, and the temperature difference between the experimental groups within 0.005° C. T...

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