Abstract
THE experimental preparations that have been utilized for bioassay of glucagon activity may be classified as follows: a) glycogenolytic response of liver slices when incubated in vitro with glucagon added to the medium, b) the reactivation of liver phosphorylase by glucagon in cell-free liver homogenates, and c) the hyperglycemic response of laboratory mammals as a result of the hormone’s glycogenolytic action in vivo. The response of liver slices in vitro as a bioassay technique was proposed by Sutherland and Cori (1948) and has had wide use. The method has been critically evaluated with standard statistical procedures by de Duve and Vuylsteke (1953). The liver homogenate method is very sensitive, Rall et al. (1957), and at the same time subject to less variation than the liver slice method, Ui et al. (1956). Thus, this method appears to be the better of the in vitro assays. The effect of exogenous glucagon on . . .
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