Abstract
In this work, we presented a long-wavelength emission fluorescent probe DCM-Cou-SePh that can discriminatively detect glutathione (GSH) and hydrogen polysulfides (H2Sn, n > 1) from green and red emission channels, respectively. With the addition of GSH, probe DCM-Cou-SePh displayed green fluorescence emission (λex/em = 430/530 nm). In the presence of H2Sn, the probe exhibited a significant fluorescence enhancement in red channel (λex/em = 560/680 nm). We also demonstrated that this probe was suitable to quantitatively detect GSH and H2Sn with low detection limits (0.12 μM for GSH, 0.19 μM for H2Sn). Furthermore, DCM-Cou-SePh can be used for sensing endogenous GSH and H2Sn in living cells by dual-color fluorescence imaging.
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