Abstract
Human cytomegalovirus (HCMV) was quantified at 6, 24, and 96 h post infection by counting the number of infected cells which were stained by the immunoperoxidase technique using monoclonal antibodies reactive to different HCMV non-structural and structural proteins. A linear dose-effect relationship was found at the three indicated times. Three rapid neutralization (Nt) assays (one for each time) were developed, using the same virus inoculum. Human sera from individuals with primary, reactivated or remote HCMV infection gave comparable Nt antibody titers with the three assays. The 6-hour rapid Nt assay appears highly suitable for determination of HCMV immune status and provides results the same day of blood collection.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Serodiagnosis and Immunotherapy in Infectious Disease
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
