Abstract
Publisher Summary This chapter provides an account of a 4S oestradiol-binding protein in uteri from mature rats that is controlled by endogenous hormones. Most experiments on the binding of oestradiol have used tissues taken from immature or long term ovariectomised animals and the relevance of such studies to the situation in mature, unovariectomised animals is not certain. The observation that protamine precipitated protein-bound oestradiol from the 4S as well as the 8S region of sucrose gradients carried out on cytoplasm derived from mature uteri prompted a study of estradiol-binding in uteri from mature animals. Analysis of the binding data obtained by the protamine method using the Scatchard plot indicated that cytoplasm from mature uteri contained one tight-binding component. This suggested that the 8S and 4S receptors detected by gradient analysis might be related to each other. If this was true, any factor influencing the 8S receptor would also affect the 4S one.
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