Abstract
Significance Transfer RNA (tRNA) transfer appropriately activated amino acids to the end of the growing peptide chain, playing significant roles in primary metabolism. Importantly, tRNA have also been shown to function outside of translation. In this report, we present the characterization of a new class of aminoacyl-tRNA (aa-tRNA)–dependent enzyme, BlsK, from Streptomyces griseochromogenes . BlsK catalyzes an aa-tRNA–dependent leucyl transfer reaction in blasticidin S biosynthesis. BlsK contains a [3Fe-4S] cluster that is necessary for its function and unprecedented in previously identified tRNA-dependent transferases. Because BlsK does not show similarity to any known functional protein, it may represent a type of aa-tRNA–dependent enzyme. As such, this work has broad implications for both tRNA-dependent and iron–sulfur–containing enzymes.
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