Abstract
DNA mismatch repair deficiency usually causes microsatellite loci length alterations during DNA replication, which is known as microsatellite instability (MSI). Recently, Next-Generation Sequencing (NGS) based MSI detection methods are very popular. However, they often show poor consistency across sequencing batches, platforms and cancer types. There is great need to develop a unified method to detect MSI status with high producibility in different NGS labs.
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