94 DUAL SCAFFOLDING SYSTEM FOR THE RECONSTRUCTION OF COMPLEX TISSUES

Abstract

You have accessJournal of UrologyTrauma/Reconstruction: Trauma & Reconstructive Surgery1 Apr 201194 DUAL SCAFFOLDING SYSTEM FOR THE RECONSTRUCTION OF COMPLEX TISSUES Mitchell Ladd, Sang Jin Lee, Anthony Atala, and James Yoo Mitchell LaddMitchell Ladd winston salem, NC More articles by this author , Sang Jin LeeSang Jin Lee winston salem, NC More articles by this author , Anthony AtalaAnthony Atala winston salem, NY More articles by this author , and James YooJames Yoo winston salem, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.159AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES While tissue engineering has had initial successes with building simple homogeneous tissues, there is an increasing demand for developing composite tissue systems that require coordinated function. One challenge in developing such tissues is designing a single scaffold that can accommodate the unique mechanical properties of the different tissue types. In this study, we developed a continuous, integrated, dual scaffolding system using a co-electrospinning fabrication technique that had regional variations in mechanical properties for the creation of complex tissues. METHODS Two different polymer solutions, 10% (w/v) poly(ε-caprolactone)/collagen and 5% (w/v) poly(L-lactide)/collagen blends with the ratio of 1:1 in weight, were simultaneously electrospun (using high voltage power at 20 kV potential) onto opposite ends of a cylindrical mandrel to create a scaffold with 3 distinct regions: a PCL/collagen side (PCL side), PLLA/collagen side (PLLA side), and a center overlap region (Fig. 1). Both solutions were delivered through a blunt tip at a constant flow rate of 1 mL/hr using a syringe pump. The distance between the syringe tip and the mandrel was 10 cm and the rate of rotation was 1000 rpm. Subsequently, the scaffolds were cross-linked with 2.5% glutaraldehyde vapor for 2 hours. Characterization of the scaffolds included ultrastructural morphology (n=3), uniaxial tensile testing (n=6), cyclic tensile testing (n=6), and stress relaxation testing (n=6). The quasi-linear viscoelastic model was used to describe the scaffold's viscoelastic behavior. The scaffold was tested for biocompatibility and seeded with muscle cells. RESULTS The results demonstrate that an integrated, dual scaffolding system can be created using co-electrospinning that is biocompatible, displays a nanofiber architecture with fiber diameters ranging from 505–606 nm, exhibits vast regional variations in mechanical properties with moduli ranging from 3,406–24,354 kPa, and withstands cyclic mechanical and stress-relaxation testing. CONCLUSIONS We have demonstrated the development of a novel, integrated, dual scaffolding system that has distinct mechanical properties within different regions of the system. The scaffold is biocompatible, accommodates muscle cells. Characterization with cyclic and stress-relaxation testing showed that the dual-scaffolding system has good mechanical properties and viscoelastic properties. This system may serve as an excellent scaffold for the formation of composite tissues. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e40 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Mitchell Ladd winston salem, NC More articles by this author Sang Jin Lee winston salem, NC More articles by this author Anthony Atala winston salem, NY More articles by this author James Yoo winston salem, NC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...