89 INFLUENCE OF DYNAMIC FLOW IN MATRIX COMPOSITION AND UROTHELIUM DIFFERENTIATION OF A TISSUE-ENGINEERED MODEL OF URETERAL SUBSTITUTE

Abstract

You have accessJournal of UrologyTrauma/Reconstruction: Trauma & Reconstructive Surgery1 Apr 201189 INFLUENCE OF DYNAMIC FLOW IN MATRIX COMPOSITION AND UROTHELIUM DIFFERENTIATION OF A TISSUE-ENGINEERED MODEL OF URETERAL SUBSTITUTE Jonathan Cloutier, Valérie Cattan, Geneviève Bernard, Gabrielle Ouellet, and Stéphane Bolduc Jonathan CloutierJonathan Cloutier Québec, Canada More articles by this author , Valérie CattanValérie Cattan Québec, Canada More articles by this author , Geneviève BernardGeneviève Bernard Québec, Canada More articles by this author , Gabrielle OuelletGabrielle Ouellet Québec, Canada More articles by this author , and Stéphane BolducStéphane Bolduc Québec, Canada More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.154AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Ureteral defects and acquired disorders often require tissue reconstruction with the use of extensive techniques. Tissue engineered ureteral bioprosthesis could overcome the small quantity of genitourinary tissue available. Mechanical stimuli are known to enhance the development and function of engineered tissue. Our objective is to compare studies of mechanical properties and the expression of different matrix and urothelium components in a human ureteral substitute cultured in dynamic and static conditions. METHODS We developed, using the self-assembly technique, a ureteral substitute composed of dermal fibroblasts and urothelial cells. Dermal fibroblasts were cultured with sodium ascorbate to form fibroblast sheets wrapped around a tubular support. After 4 weeks of maturation, urothelial cells were seeded inside the luminal area of our substitutes. Ureteral substitutes were subjected to dynamic flow for 1 and 2 weeks and were compared to static conditions. The expression of matrix and urothelium components (collagen I, elastine and others) was studied using immunofluorescence (IF) and reverse transcription polymerase chain reaction (RT-PCR). Permeability and mechanical resistance was determined by the permeability test using the Carbon-14 radioactive isotope and burst pressure. RESULTS The dynamic flow showed a pseudostratified urothelium in comparison to the static. Dynamic flow tended to increase matrix components (elastin and collagen I) on IF and mRNA expression. Urothelium differentiation was illustrated on IF after 14 days of perfusion. The permeability test demonstrated superior impermeability in the dynamic group (2weeks>1week) as compared to the static. Mechanical resistance demonstrated equal resistance between dynamic and static but was more enhanced than the native urothelium. CONCLUSIONS We are the first group to demonstrate a urothelial differentiation with dynamic flow. Our tissue-engineered ureteral tissue presents interesting impermeability, resistance and expresses the main extracellular matrix components present in native ureteral tissue. Our tissue engineered ureteral model cultured with dynamic condition presents biological and mechanical properties encouraging for clinical use. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e38-e39 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jonathan Cloutier Québec, Canada More articles by this author Valérie Cattan Québec, Canada More articles by this author Geneviève Bernard Québec, Canada More articles by this author Gabrielle Ouellet Québec, Canada More articles by this author Stéphane Bolduc Québec, Canada More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...