Abstract
Publisher Summary Nuclear magnetic resonance (NMR) microscopy refers to the generation of NMR images with micrometer-scale resolution within the pixel plane. As a method for noninvasive three-dimensional microscopy, it has an advantage over light microscopy in opaque dielectric samples and in obtaining information beyond the 500-nm limit of confocal microscopy. At this resolution, NMR microscopy does not directly compete with other microscopy methods for the elucidation of tissue structure; instead, it is sensitive to the details of molecular structure, chemical dynamics, and intermediary metabolism. NMR spectroscopy has been successful with respect to the elucidation of chemical structure and metabolism. It can give spatial information about these chemical and dynamic events. NMR microscopy is noninvasive and nondestructive, as long as the samples fit within the confines of the receiver coils. There are no known significant biological effects of the low-frequency electromagnetic fields used in the generation of NMR images. NMR microscopy is well suited to the examination of biological samples under physiological conditions of temperature, pH, ionic strength, and atmospheric pressure. These attributes of NMR have led to the explosive growth in the use of NMR imaging in medicine.
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