Abstract
Publisher Summary This chapter discusses the inhibitors of mitochondrial electron transport. Certain inhibitors act in the cytochrome b–c 1 region and in the flavoprotein region. This chapter provides an overview on the inhibitors of NADH oxidation. Rotenone is a compound that inhibits the oxidation of NADH-linked substrates in mitochondrial and submitochondrial preparations from animal tissues. Maximal inhibition is 99%. This is attained by treating submitochondrial particles (ETP) with 0.4 nmol of rotenone per milligram of protein. Rotenone inhibition shows a pronounced lag period. In submitochondrial particles, this lag is minimized by preincubation of the particles with NADH. Besides being bound at the specific sites, it is primarily responsible for inhibiting electron transport between NADH dehydrogenase and coenzyme Q, rotenone is also bound at various unspecific sites. Mitochondria and particles derived from them act as scavengers of rotenone and rotenoids. Procedures for minimizing unspecific binding of rotenone and resultant inhibition are discussed in the chapter. The chapter also reviews inhibitors of succinate oxidation. 2-Thenoyltrifluoroacetone is a selective inhibitor of succinate dehydrogenase in membrane preparations, acting at the dehydrogenase coenzyme Q junction.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
