Abstract
<p class="Abstract">The present study was performed to examine the effect of 5-bromo-3-(3-hydroxyprop-1-ynyl)-2H-pyran-2-one (BHP) on the rate of cell proliferation and apoptosis induction in the PC3, human prostate carcinoma cell line. The cell viability was assayed by using sulphorhodamine B staining and apoptosis by annexin V and flow cytometry analyses. The results revealed that BHP treatment in PC3 cells caused a significant reduction in the rate of cell proliferation in dose- and time-dependent manner. Compared to the un-treated cells, the formation of HUVEC tubes was markedly inhibited on treatment with BHP at a concentration of 30 µM. Further investigation revealed the expression of HMGB1, IL-6 and IL-8, pro-inflammatory cytokines was also inhibited on treatment with BHP. Therefore, BHP treatment plays an important role in inducing apoptosis in the prostrate cells and can be of therapeutic value for the prostate cancer treatment.</p><p> </p>
Highlights
Prostate cancer, one of the frequent detected causes of mortality in men, accounts for more than 25, 000 deaths in the United States alone every year (Jemal et al, 2010)
The present study was performed to investigate the effect of 5-bromo-3-(3-hydroxyprop-1-ynyl)-2H-pyran2-one (BHP), an analog of 2-pyrone, in the inhibition of cell growth and induction of apoptosis in the prostate cancer cells
The results from the quantitative real-time RT-PCR analysis revealed that BHP treatment in PC3 cells decreased the HMGB1, IL-6, and IL-8 expression levels and promoted the NAG-1 expression compared to the control cells (Figure 5)
Summary
One of the frequent detected causes of mortality in men, accounts for more than 25, 000 deaths in the United States alone every year (Jemal et al, 2010). Angiogenesis in prostate carcinoma cells influences the expression levels of p65, VEGF and MMP-9 Another important factor with role in the progress of cancer inflammation which is initiated and regulated by cytokines and chemokines (Lu et al, 2006; Coussens and Werb, 2002). 2-Pyrones, the cyclic 6-membered esters are the natural isolates obtained from the extracts of plants, bacteria and other animals by using conventional chromatography (Jensen and Fenical, 1994; Faulkner, 2001). Their role in providing protection to the organisms against various other attackers is well established (McGlacken and Fairlamb, 2005). The present study was performed to investigate the effect of 5-bromo-3-(3-hydroxyprop-1-ynyl)-2H-pyran2-one (BHP), an analog of 2-pyrone, in the inhibition of cell growth and induction of apoptosis in the prostate cancer cells
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
