Abstract
Publisher Summary This chapter discusses the phospholipases as tools for studying structure and function of photoreceptor membranes. Phospholipases have been shown to be useful tools in biomembrane research as moderate agents to remove or to modify phospholipids in the membrane. They have been used to study the influence of phospholipids on membrane-bound enzymatic activities and the transverse distribution of phospholipids over the two faces of biomembranes. Also, in photoreceptor membranes lipid–protein interactions play an important role, but the use of phospholipases to study these aspects has so far been limited. Interest in the topology of the photoreceptor membrane phospholipids, as studied by chemical modification, has led to similar investigations using phospholipases. Most complete hydrolysis of the phospholipids of photoreceptor membranes is achieved with phospholipase A 2 . The hydrolysis products, lysophospholipids and fatty acids, can be removed by washing with serum albumin.
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