Abstract
Publisher Summary The membrane-free cytosol of pig brain contains glycolipid transfer protein (GL-TP) that stimulates the transfer of various glycosphingolipids and glyceroglycolipids among membranes in vitro. The protein has been purified to homogeneity from this source. The GL-TP from pig brain functions as a specific carrier of glycolipids most likely by forming a 1 : 1 molecular complex. Under conditions where one of the membranes lacks glycolipids, GL-TP has been found to catalyze a net transfer reaction. The chapter describes the purification of GL-TP from pig brain. GL-TP has also been partially purified from rat brain and several cell lines of rat, mouse, and human origin. Antisera raised against pig GL-TP in rabbits are cross-reactive with rat, mouse, and human GL-TP.
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