Abstract
This chapter discusses quantitative transmission electron microscopy for the determination of mass-molecular weight of viruses. The determination of the mass of microscopic objects by quantitative transmission electron microscopy is a simple but powerful procedure. In addition to allowing the observation of the general features of size and shape, the important property of mass is quickly and directly ascertained. In assigning an average mass value to a virus, quantitative electron microscopy attains the same experimental goals as other procedures for determining molecular weight. In deriving the mass-molecular weight of viruses, the electron microscope is used as an analytical balance covering the entire weight range of all the viruses so far reported in the literature. Its ease of operation, coupled with the critical features of time and the requirement of only small quantities of virus, ranks the electron microscope as a premier research instrument for this important aspect of virology.
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