4-Amino-3 H-pyrimidin-2-one (“isocytosine”) is a short-lived non-radical intermediate formed in the pulse radiolysis of cytosine in aqueous solution

Abstract

In the pulse radiolysis of 2′-deoxycytidine (dCyd) in N 2O-saturated solutions containing 0.5 M tertiary butanol to completely scavenge the water radicals, a short-lived intermediate ( λ max = 287 nm ) is observed by UV spectroscopy which is attributed to dCydH +, generated in the reaction of dCyd with H + formed during the pulse. By reacting with OH −, which is formed in the pulse in amounts matching that of H +, this intermediate disappears in the μs time range without a change of the spectrum. Similarly, cytosine (Cyt) gives rise to CytH + which, in contrast, in part transforms into another species ( λ max = 286 nm ) which can be assigned to isocytosine 1, 4-amino-3 H-pyrimidin-2-one, a tautomer of Cyt which is formed by two routes, (i) deprotonation of CytH + at N(1) by OH − and (ii) deprotonation of Cyt and reprotonation of the Cyt anion by water at N(3). Compared to Cyt, 1 is richer in Gibbs’ free enthalpy by 14 kJ mol −1. Its presence in low equilibrium concentrations has also been observed by conventional UV spectroscopy, making use of the increase of its equilibrium concentration with increasing temperature. From these data, an absorption coefficient of 3.3×10 4 dm 3 mol −1 cm −1 at 286 nm has been calculated. Supporting quantum chemical calculations are also reported.